Two percent sodium chloride is required for susceptibility testing of staphylococci with oxacillin when using agar-based dilution methods
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چکیده
منابع مشابه
Detection with Agar Dilution Susceptibility Testing
A dye reduction method for determining a definitive endpoint with agar dilution susceptibility testing has been developed. Bacterial growth was determined by applying to the inoculum spot a dye solution containing 2-(p-iodophenyl)-3-(pnitrophenyl)-5-phenyltetrazolium chloride and phenazine methosulfate. Viable colonies reduced the tetrazolium salt to a visible red color within 3 to 5 min. The m...
متن کاملMethicillin susceptibility testing of staphylococci by Etest and comparison with agar dilution and mecA detection.
The susceptibility to methicillin of 44 Staphylococcus aureus and 120 coagulase-negative staphylococci (CNS) was determined by Etest, agar dilution and presence of the mecA gene. There was agreement between the results of all methods when testing S. aureus. However, discrepancies occurred with CNS when cultural methods were compared with presence of the mecA gene. mecA-positive isolates tested ...
متن کاملClinical validation for oxacillin susceptibility testing of coagulase negative staphylococci.
Oxacillin susceptibility of coagulase negative staphylococci as assessed by conventional methods was confirmed by a modified Etest method, extended to detect heteroresistance. Verification of susceptibility was followed by successful treatment for six consecutive children with deep seated infections. Physicians' trust in such a validated method will contribute to the appropriate use of antibiot...
متن کاملComparison of M.I.C.E. and Etest with CLSI Agar Dilution for Antimicrobial Susceptibility Testing against Oxacillin-Resistant Staphylococcus spp
OBJECTIVE The main objective of this study was to comparatively evaluate the performance of M.I.C.E. and Etest methodologies to that of agar dilution for determining the antimicrobial susceptibility profile of oxacillin-resistant Staphylococcus spp. METHODS A total of 100 oxacillin-resistant Staphylococcus spp. isolates were collected from hospitalized patients at a teaching hospital. Antimic...
متن کاملAgar dilution method for susceptibility testing of Neisseria gonorrhoeae.
The antibiotic susceptibilities of Neisseria gonorrhoeae isolates obtained from patients attending a clinic for sexually transmitted diseases in Tucumán, Argentina, were determined by the agar dilution method (MIC). 3.5% of the isolates produced beta-lactamase. A total of 96.5% of beta-lactamase negative isolates tested were susceptible to penicillin (MIC < or = 2 micrograms/ml-1); 14.03% of th...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.10.2683-2688.1993